日本血吸虫ELAV-like 2基因的克隆、表达及功能分析

doi:10.11843/j.issn.0366-6964.2018.09.020

Abstract

Abstract:

Schistosomiasis is a globally important helminthic disease of humans and animals,eggs produced by the mature female worms are the main factor that cause pathological damage and disease transmission. The study on controlling female reproductive development has great significance in controlling schistosomiasis. In this study, the full-length of SjELAV-like 2 gene sequence was attained by 5'-RACE and 3'-RACE. The SjELAV-like 2 coding sequence was successfully cloned and expressed, and the polyclonal antibody was produced. Western blotting analysis showed that the protein has good reactionogenicity. RT-qPCR analysis revealed that the gene had the highest expression in schistosomula and tended to be stable after maturation. The expression level of SjELAV-like 2 in the female was significantly higher than that in the male. Its expression in each periods of the male remained maintenance level. Immunohistochemical results indicated that the protein was mainly located in the tegument of Schistosoma japonicum in adult females. RNAi interference technology was employed to study the biological function of SjELAV-like 2 gene. RT-qPCR results showed that RNAi could effectively reduce the expression of SjELAV-like 2 gene in the parasites. The long-term RNAi also reduced the hatching rates about 75.56% (P<0.01), and reduced the liver egg burden about 39.67% (P<0.01). The scanning electron microscope showsed that there were more vesicular protrusions on the tegumental tissues of siRNA interference group worms compared with the negative control. These observations implied that SjELAV-like 2 has important influence on growth and development of Schistosoma japonicum.

CLC Number:

S852.735

ZHANG Yuan-yuan, XU Rong, HE Chuan-chuan, LI Xiao-chun, CHENG Gui-feng, GUO Lu, LIU Jin-ming, LI Hao, GU Shao-peng, JIN Ya-mei. Cloning,Expression and Functional Analysis of ELAV-like 2 Gene of Schistosoma japonicum[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1987-1997.

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Cloning,Expression and Functional Analysis of ELAV-like 2 Gene of Schistosoma japonicum

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